Brown Adipose Tissue

1. Brown Adipose Tissue: A Short Historical Perspective

Paul Trayhurn

2. Brown Fat Anatomy in Humans and Rodents

Georgia Colleluori, Jessica Perugini, Angelica Di Vincenzo, Martina Senzacqua, Antonio Giordano, and Saverio Cinti

3. Analysis of Thermogenesis Experiments with CalR

Marissa D. Cortopassi, Deepti Ramachandran, William B. Rubio, Daniel Hochbaum, Bernardo L. Sabatini, and Alexander S. Banks

4. A Clearing Method for Three-Dimensional Imaging of Adipose Tissue

Zeran Lin, Jingyi Chi, and Paul Cohen

5. Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron

Kong Y. Chen, Robert J. Brychta, Nikita S. Israni, Alex Jiang, Hannah J. Lea, Taylor N. Lentz, Annie E. Pierce, and Aaron M. Cypess

6. Fluorescent and Luminescent Methods to Detect Lipolysis

Emilio P. Mottillo and James G. Granneman

7. Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice

Markus Heine, Carlotta Corban, and Joerg Heeren

8. Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes

Su Myung Jung, Johnny Le, Will G. Doxsey, John A. Haley, Grace Park, David A. Guertin, and Cholsoon Jang

9. Activation of UCP1-Independent Ca²⁺ Cycling Thermogenesis by Wireless Optogenetics

Kenji Ikeda, Kazuki Tajima, Yuji Tanabe, Ada S.Y. Poon, and Shingo Kajimura

10. Measurement of Futile Creatine Cycling Using Respirometry

Janane F. Rahbani, Edward T. Chouchani, Bruce M. Spiegelman, and Lawrence Kazak

11. Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes

Anne Loft, Maja Worm Andersen, Jesper Grud Skat Madsen, and Susanne Mandrup

12. Diet-Induced Thermogenesis: Principles and Pitfalls

Jan Nedergaard and Barbara Cannon

13. Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice

Rajib Mukherjee and Joan Sanchez-Gurmaches

14. Isolation and Characterization of Human Brown Adipocytes

Camilla Scheele, Tora Ida Henriksen, and S ren Nielsen

15. Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The ’Goldilocks Solution’

Min Li and John R. Speakman

16. Signaling Lipidomic Analysis of Thermogenic Adipocytes

Sean D. Kodani, Valerie Bussberg, Niven R. Narain, Michael A. Kiebish, and Yu-Hua Tseng

17.